Multimembrane dot-blotting: a cost-effective tool for proteome analysis
نویسندگان
چکیده
منابع مشابه
Multimembrane dot-blotting: a cost-effective tool for proteome analysis.
The molecular profiles of protein expression from hundreds of cell lysates can be determined in a high-throughput manner by using fluorescent bead technologies, enzyme-linked immunosorbent assays (ELISAs), and protein microarrays. Although powerful, these tools are costly and technically challenging and thus have limited accessibility for many research groups. We propose a modification of tradi...
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Dot and slot blotting are simple techniques for immobilizing bulk unfractionated DNA on a nitrocellulose or nylon membrane. Hybridization analysis can then be carried out to determine the relative abundance of target sequences in the blotted DNA preparations. Dot and slot blots differ only in the geometry of the blot, a series of spots giving a hybridization pattern that is amenable to analysis...
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Definitive hosts of the Echinococcus granulosus (E. granulosus ) parasite are carnivores such as dogs,wolves and foxes. Detection of this parasite through faecal examination is not possible. In this study, dotblotting test for E. granulosus -specific coproantigens has been evaluated in dog. Three 2–3-month-old puppies were treated with piperazine and then faecal samples were collected as pre-in...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2004
ISSN: 0736-6205,1940-9818
DOI: 10.2144/04366pf01